Automating NGS with the Illumina DNA Prep Kit
App note: Automating DNA library prep with 3 different kits
If you’re looking to automate your NGS library prep, you want to be sure that your chosen liquid handler can work with your reagents and workflow. Check out this app note demonstrating methods of automating NGS library prep with 3 kits, including the Illumina DNA Prep Kit on the OT-2.
Find out if automating NGS is right for you.
DOWNLOAD EBOOKINTRODUCTION
Why automate NGS?
NGS is a powerful tool, but many parts of the NGS workflow are complex, require a lot of repetitive pipetting and are prone to errors. Labs are increasingly turning to automation to help with their NGS workflows. Automating your NGS workflow can increase efficiency, reduce errors and save hands-on time.
Automating library prep, normalization and pooling saves total workflow time and hands-on time
NGS workflow from nucleic acid extraction through to sequencing prep, with two different example NGS library prep workflows shown
WORKSTATION
Automate your NGS workflow on the OT-2
The OT-2 can automate your library prep and pre-sequencing workflows using any leading reagent or magnetic bead-based system on the market. Explore our popular NGS workstation, which includes all the hardware modules and consumables you need to start automating NGS immediately.
Library prep on the OT-2
Quantification, normalization and pooling on the OT-2
"We automated much of the [OCTOPUS plasmid sequencing] protocol using the open-source Opentrons OT-2 liquid handling system and reduced the necessary hands-on time by more than 70%."
Bryan Jiang
Research Associate,
Octant
Research Spotlights
bioRxiv
Microbial WGS using Illumina library prep on the OT-2
A low-cost, high-throughput bacterial isolation and genome sequencing method for characterizing genomic diversity within gut microbiota
BMC Genomics
Choosing a liquid handler for your genomics lab
A review article outlining considerations for genomics labs looking to automate their NGS workflows
Resources
Explore application data and protocols for different NGS workflow reagents on the OT-2 in the table below. Don’t see your preferred NGS reagents here? Our protocol specialists create protocols for NGS library prep regularly. Book a virtual demo to discuss your workflow needs with an expert.
*Verification categories:
Opentrons = has been verified by the Opentrons Team, who verify that the scientific results are as expected.
Partner = has been verified by our reagent partner, who verifies that the scientific results are as expected.
Community = protocol is in use by members of the Opentrons community.