Automating 96-sample library prep with the new Opentrons Flex High-Throughput NGS Workstation

Automated liquid handling has become a necessity for reliably scaling high-throughput Next-Generation Sequencing (NGS) library preparation. The new Opentrons Flex High-Throughput NGS Workstation includes all required components for fully automated NGS library preparation, including the Opentrons Flex Stacker, which automates replenishment of pipette tips and labware onto the robot deck. Here, we present high-yielding and uniform libraries with coefficient of variation (CV) as low as 9%.