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Flexible automation of DNA assembly with OT-2 lab robots and DIVA


Molecular cloning remains a manual, low throughput and unreliable process in most cases and limits our ability to advance the science and engineering of biology. Here, we present AssemblyTron, an open-source Python package to integrate DNA assembly design outputs with build implementation in Opentrons liquid handling robotics with minimal human intervention. We will introduce AssemblyTron, its capabilities, installation, and workflow from construct design (using DIVA: DNA Design, Implementation, and Validation Automation Software) through primer design, PCR, and DNA assembly via homology dependent or Golden Gate assembly methods. 

Sign up to access this 60-minute webinar to learn:
  • DNA assembly capabilities of AssemblyTron and OT-2
  • Installing AssemblyTron on your OT-2
  • The AssemblyTron workflow
  • Future features of AssemblyTron 

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John Bryant

John Bryant

PhD Candidate, Virginia Tech

John is a PhD student in Biological Systems Engineering at Virginia Tech.  John builds software packages for automating synthetic biology with liquid handling robotics. He also designs custom DNA parts that integrate with existing modular cloning toolkits to facilitate rapid multigene plasmid assembly. 

Clay Wright

Clay Wright

Assistant Professor, Virginia Tech

Prior to studying protein engineering, Clay directed evolution, and synthetic biology as a PhD student in Chemical and Biomolecular Engineering at Johns Hopkins University. Clay was a postdoctoral fellow in Biology and Electrical Engineering at University of Washington, prior to starting his own lab at Virginia Tech in 2019. The Wright Plant Synthetic Biology Lab studies and engineers how complex, dynamic gene networks determine cell fate and how robustness, plasticity, and evolution of these networks are linked across cells, tissues, and organisms.