Webinar
How to use CSV Files for Complex Pipetting Patterns using Opentrons Flex
On-demand
Watch this webinar to see a live demo of how to use an Opentrons Flex to complete complex pipetting tasks. While pipetting the same volume into every row or column on a plate is time-consuming and error prone enough, irregular sequences of wells or volumes are the most common causes of errors in manual pipetting. Having to find individual wells frequently leads to wrong-well errors, however the added challenge of changing pipette volume for each individual well results in a much greater level of risk for mistakes.
An Opentrons Flex won’t lose its place and use the wrong well, nor will it pipette the wrong volume, even if this changes every well.
The easiest way to specify these complex pipetting patterns is to use a .csv file, most commonly exported from a spreadsheet. This webinar will demonstrate how to format and export a .csv file for use on an Opentrons Flex, and also how to upload and run that file using the robot touchscreen.
Some of the most common uses for .csv files to direct complex pipetting patterns include:
- Concentration normalization, where volumes of both sample and diluent change for every well
- Cherrypicking, where only select wells from a plate are transferred to a new plate, for example picking positive clones
- Pooling, where select wells are pooled to a single well in a new plate, for example collecting chromatography fractions
Who should watch:
- Anybody whose pipetting pattern isn’t a series of adjacent wells
- Anybody who has to pipette different volumes into each well
- Opentrons users already use .csv files and would like to see the new improved upload procedure
Access now
Speakers
Jon Martinez
Direct of Sales, Opentrons Labworks
Nick Diehl
Software Engineer - Protocol Authorship, Opentrons Labworks